NASE is currently being integrated into OpenMS. Until the next OpenMS release, a custom installer is provided:

>>> Download the 64bit installers for Win, Linux, OSX <<<

Note: on some Windows PCs, the installer may report an error about being unable to install a Visual Studio redistributable package. This warning can be ignored.


  • HCD data acquired on a high-resolution MS
  • Developed and tested on orbitrap instruments
  • MS/MS need to be centroided (either on acquisition, conversion, or in a workflow using the TOPP tool PeakPickerHiRes)

Raw files:

The mass spectrometry data have been deposited to the ProteomeXchange Consortium ( via the PRIDE partner repository with the dataset identifier PXD012094, PXD012095, PXD012097  

Example workflow:

Download example TOPPAS workflow 


Preprint available on bioRxiv: